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產品名稱：重組人類血液濾液CC趨化因子-1 / CCL14蛋白
產品型號：rHuHCC-1/CCL14
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人類血液濾液CC趨化因子-1 / CCL14蛋白與其它公司提供的重組蛋白不同，rHuHCC-1/CCL14蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類血液濾液CC趨化因子-1 / CCL14蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人類血液濾液CC趨化因子-1 / CCL14蛋白

Synonyms HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14

Species Human

Accession Q16627

GeneID 6358

Source Escherichia coli.

Molecular Weight 重組人類血液濾液CC趨化因子-1 / CCL14蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人類血液濾液CC趨化因子-1 / CCL14蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuHCC-1/CCL14 as determined by LAL method.

Reconstitution 重組人類血液濾液CC趨化因子-1 / CCL14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8°C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人類血液濾液CC趨化因子-1 / CCL14蛋白

Reference 1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9.
2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8.
3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13.

Background Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma.

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Synonyms	HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14
Species	Human
Accession	Q16627
GeneID	6358
Source	Escherichia coli.
Molecular Weight	重組人類血液濾液CC趨化因子-1 / CCL14蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人類血液濾液CC趨化因子-1 / CCL14蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuHCC-1/CCL14 as determined by LAL method.
Reconstitution	重組人類血液濾液CC趨化因子-1 / CCL14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類血液濾液CC趨化因子-1 / CCL14蛋白
Reference	1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9. 2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8. 3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13.
Background	Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma.

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產品名稱：重組人類血液濾液CC趨化因子-1 / CCL14蛋白