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產品詳情
  • 產品名稱:重組鼠白介素-19蛋白

  • 產品型號:rMuIL-19
  • 產品廠商:KALANG
  • 產品文檔:
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簡單介紹:
重組鼠白介素-19蛋白與其它公司提供的重組蛋白不同,rMuIL-19蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠白介素-19蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組鼠白介素-19蛋白

Species Murine
Accession Q8CJ70
GeneID 329244
Unigene Mm.131480.
Source Escherichia coli.
Molecular Weight 重組鼠白介素-19蛋白Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
AA Sequence LRRCLISVDM RLIEKSFHEI KRAMQTKDTF KNVTILSLEN LRSIKPGDVC CMTNNLLTFY RDRVFQDHQE RSLEVLRRIS SIANSFLCVQ KSLERCQVHR QCNCSQEATN ATRIIHDNYN QLEVSSAALK SLGELNILLA WIDRNHLETP AA
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組鼠白介素-19蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 × 106 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 3 % Trehalose.
Endotoxin Less than 0.1 EU/μg of rMuIL-19 as determined by LAL method.
Reconstitution 重組鼠白介素-19蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠白介素-19蛋白
Background Interleukin-19 (IL-19) belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis.

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