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產品名稱：重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白
產品型號：rRtEotaxin-2/CCL24
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白與其它公司提供的重組蛋白不同，rRtEotaxin-2/CCL24蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白

Synonyms CK-beta-6, MPIF-2, Small-inducible Cytokine A24

Species Rat

Source Escherichia coli.

Molecular Weight Approximately 10.5 kDa, a single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat Eotaxin-2.

Quantity 5μg/20μg/1000μg

AA Sequence 重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白MPTGSVTIPS SCCVTFISKK IPVNRVISYQ LANGSICPKA GVIFITKKGH KICTDPKLPW VQKHIKNLDA KRNQPSEGAK ALGPKFVIQK LRGNSTKV

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml.

Physical Appearance 重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rRtEotaxin-2/CCL24 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白

SDS-PAGE

Reference 1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5.
2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79.
3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.

Background CCL24, also named MPIF-2, Eotaxin-2 and Ckβ6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

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Synonyms	CK-beta-6, MPIF-2, Small-inducible Cytokine A24
Species	Rat
Source	Escherichia coli.
Molecular Weight	Approximately 10.5 kDa, a single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat Eotaxin-2.
Quantity	5μg/20μg/1000μg
AA Sequence	重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白MPTGSVTIPS SCCVTFISKK IPVNRVISYQ LANGSICPKA GVIFITKKGH KICTDPKLPW VQKHIKNLDA KRNQPSEGAK ALGPKFVIQK LRGNSTKV
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml.
Physical Appearance	重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rRtEotaxin-2/CCL24 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白
SDS-PAGE
Reference	1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5. 2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79. 3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.
Background	CCL24, also named MPIF-2, Eotaxin-2 and Ckβ6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

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產品名稱：重組大鼠嗜酸性粒細胞趨化因子-2 / CCL24蛋白