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產品名稱：重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白
產品型號：rHuEotaxin-2/CCL24
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白與其它公司提供的重組蛋白不同，rHuEotaxin-2/CCL24蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白

Synonyms CK-beta-6, MPIF-2, Small-inducible Cytokine A24

Species Human

Accession O00175

GeneID 6369

Source 重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白Escherichia coli.

Molecular Weight Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuEotaxin-2/CCL24 as determined by LAL method.

Reconstitution 重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白

Reference 1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5.
2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79.
3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.

Background CCL24 is a secreted protein, encoded by CCL24 gene in humans located on Chr.7, and produced by activated monocytes and T lymphocytes. CCL24 has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

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Synonyms	CK-beta-6, MPIF-2, Small-inducible Cytokine A24
Species	Human
Accession	O00175
GeneID	6369
Source	重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白Escherichia coli.
Molecular Weight	Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuEotaxin-2/CCL24 as determined by LAL method.
Reconstitution	重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白
Reference	1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5. 2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79. 3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.
Background	CCL24 is a secreted protein, encoded by CCL24 gene in humans located on Chr.7, and produced by activated monocytes and T lymphocytes. CCL24 has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

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產品名稱：重組人嗜酸性粒細胞趨化因子-2 / CCL24蛋白