產(chǎn)品詳情
簡(jiǎn)單介紹：
重組人成纖維細(xì)胞生長(zhǎng)因子4蛋白與其它公司提供的重組蛋白不同，rHuFGF-4蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組人成纖維細(xì)胞生長(zhǎng)因子4蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組人成纖維細(xì)胞生長(zhǎng)因子4蛋白
| Synonyms | HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3 |
| Species | Human |
| Accession | P08620 |
| GeneID | 2249 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人成纖維細(xì)胞生長(zhǎng)因子4蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
| Purity | 重組人成纖維細(xì)胞生長(zhǎng)因子4蛋白> 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl. |
| Endotoxin | 重組人成纖維細(xì)胞生長(zhǎng)因子4蛋白Less than 1 EU/μg of rHuFGF-4 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人成纖維細(xì)胞生長(zhǎng)因子4蛋白 | |
| SDS-PAGE |
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| Reference |
1. Galland F, Stefanova M, Lafage M, et al. 1992. Cytogenet Cell Genet. 60:114-6. 2. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 3. Boulet AM, Capecchi MR. 2012. Dev Biol. 371:235-45. |
| Background | FGF-4, also named FGF-K and K-FGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF-4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF-4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family. |
